(Prime each pump head separately. Leak at fittings, purge valve, active inlet If there are still signs of a. 5 0. Residues or contaminations may block filters or capillaries. Figure 6: Pump head with purge valve and outlet to autosampler. Select this procedure when you want to prime and purge any 1525 Binary Series. In the right part of the image, the glass piston is. Basic HPLC System Components. Sonication: ultrasonic baths as a stand-alone. 1 Purpose 4 1. 7:30. The manual. This instalment provides an overview for analytical-scale HPLC pumps, including their requirements, modern designs,. For modular autosamplers purge from front panel. Check for leaks and perform PM. This video explains the reasons for pump priming. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. You may need to remove and sonicate it. g. closed. BEFORE WORKING WITH THIS ANALYTICAL HPLC 1. 2:32. 결과 및 정리. 2. From my little experience i learnt that it may be due to air trapped in. It is column chromatography. Pump 100% isopropanol through the pump for 3 minutes. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . 02 min SD (whichever is larger) of the set flow rate. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. To purge the 2414 via Empower, use the Purge. And, remember, if you have a dual-piston pump, you have two piston seals to replace. It is Liquid Chromatography. ), prime system if necessary. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. Figure 6: Pump head with purge valve and outlet to autosampler. The same problem has been observed with the Shimadzu LC-2040 C3D plus / RF HPLC chain. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. Open prime/purge valve (in the case of Chrom Tech pumps, you turn the knob counterclockwise one to two turns. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. Meet Your New Lab Ally - the Alliance iS HPLC System. Prime the pump with degassed solvent. This video shows the proper procedure to safely remove and replace the plunger seal on the Shimadzu LC-40 Series Nexera pump. Among the various technologies developed for chromatography,. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. between the degasser and the column. try to locate source of particles (sample, eluents, pump mechanics, injection valve). 2 Set the flow of the pump at 1ml/min. with a new bottle of HPLC-grade water. 5. Page 103 • Operation Figure 29: Dwell volume. Varian Modular Analytical HPLC Systems Figure 1. Therefore, the term "thePurge pump and attempt to clean check valves. If the reservoir runs dry in your HPLC pump you will draw air into the pump, but the pump will not pump air through the system. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on. See Reference 1 for interactive video. If problem still persists, contact system manufacturer. Purge each mobile phase that will be used by selecting “Purge” on the PumpModule tab on Chromeleon. 2. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersLC-40D XR Specifications. Focus on the column's inlet: After the initial flush, reduce the flow rate to 1 mL/min or so and close the purge valve until no more bubbles emerge. Please refer to the section “Exchanging the Purge Valve Frit or the Purge Valve” on page 126 in this manual. Next, close the vent valve to slightly pressurize the gas in contact with the liquid. 01. Leak at pump 18 Selectivity Differences in selectivity 19 Baseline Disturbance at void time 20. This pump also facilitates seamless method transfer using ISET and automated buffer blending. L/mni for 5mni . This video shows the operation a HPLC purge valve. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. If you have an Agilent 1260 Infinity Isocratic or Quaternary Pump, remove the tubing to your passive inlet valve (PIV). ), prime system if necessary. Versatile and Flexible The Thermo Scientific™ Dionex™ UltiMate 3000 pump family offers the most complete choice in the industry. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. ID stainless steel tubing kit • Galaxie Chromatography Software-0. Once the air is removed, close the valve and start flow. Purification Solvent Management. The new 1290 Infinity II LC power range has a high instrument efficiency, allowing you to run any HPLC and UHPLC method. In general, the degasser should be left on, and all solvent lines including the unused ones should be filled with solvents. A damping unit is connected between the two piston chambers. Move to step 2. *Run to run RT variation of ±0. The manual covers installation, operation,. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Open the Purge Valve of your pump and purge each solvent line, one by one, with HPLC-quality Isopropanol (IPA). Pump a 20% nitric acid/water solution through the pump for 3 minutes. Priming and Purging the 1525 Binary HPLC Pump. The 1290 Infinity II High Speed Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. How do i manually prime/purge it? Because there are some air bubbles, and if they get inside the needle, its gonna be bad news bears ( i think). isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC). HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. 2 shows the flow line during LC-2010 analysis. Request a Certificate. 23 MPa. P680 HPLC PumpsPurge pump at high flow rate (e. Manual - isocratic HPLC System G1380AA standard configuration • G1310A isocratic pump with solvent cabinet • G1328A manual injector (MI) • G1314A variable wavelength detector (VWD) with standard flow cell (10 mm path length, 14 µl volume, 40 bar maximum pressure) • G2220AA 2D-Value Solution ChemStation other modules that can be addedFailure to use fresh clean pickup filters in your HPLC may lead to total contamination of the flow path (column, pump, A/S and detector(s)). If there is no pressure drop, the blockage is. DO NOT PUMP ANY FLUSHING SOLVENT THROUGH THE SEAL WASH LINE, OR INJECTION NEEDLE LINES. From my little experience i learnt that it may be due to air trapped in. Blockages can also be in front of the degasser, meaning the solvent inlet filters in the solvent bottle. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. To start purging, press the "Purge" bottom on both LC-20AD and SIL-20AC. Purge valve: Reciprocating piston pumps do not work if there is air in the lines, which can be the case after changing a solvent reservoir, or leaving the pump off. High Priced Liquid Chromatography. Popular answers (1) There can be several reasons for the pressure drops you observe: air could still be present in the system after your recent repair (i. A damping unit is connected between the two plunger chambers. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. 5 kgfcm -2, for example) for 10 to 30 minutes to purge the air from the mobile phase. 02-0. A damping unit is connected between the two piston chambers. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. Some pumps will require to manually open the purge valve and in others it is done via the software. The system must be primed to remove air bubbles and for the pump to function properly. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Blockage of. Vacuum degassing removes more than 60% of dissolved air. L/mni for 5mni . The purge valve, acting as an essential component of your vehicle’s Evaporative Emissions Control System (EVAP), plays a vital role in reducing harmful emissions and maintaining fuel efficiency. Treatment 2: Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. Replace the filter frits if necessary. UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. Maintenance Kits for UltiMate™ 3000 Pumps. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. Rough pumps stay on so the system remains under vacuum. The flow diagram to the right explains this function. [][]Fill up & attach Labels of the solvent reservoirs properly. 02 to 0. PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. operating pressure (9,99 ml/min max. Residues or contaminations may block filters or capillaries. Draw approximately 20 mL of fluid. 3. However, in order to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation. 1:55. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. Perform sample clean-up (i. The reduction in condensation risk can allow the pump to achieve its vapor duty at. From my little experience i learnt that it may be due to air trapped in. 3601-EN-5. The 1260 Infinity II Quaternary Pump is a gradient pump that uses low-pressure mixing for UHPLC gradient formation and solvent blending from up to four solvents. Place the solvent bottle back into LC eluent. † Automated instrument features like switching mixer in/out, automaticVarian Modular Analytical HPLC Systems Figure 1. (Prime each pump head separately. Power off the pump and remove the waste tubing from the purge valve. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. The 1290 Infinity II Flexible Pump is a quaternary pump that provides UHPLC gradient formation and solvent blending from up to four solvents. 2 Push the “conc” button on LC20AD. , 5-10 mL/min. HPLC Principle. See your pump modules user manual for detailed instructions on re-assembly. 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. Detector – responds to the separated analytes emerging from the HPLC. PENDAHULUAN Penelitia n yang berkaitan dengan tumbuhan berguna Indonesia. Easy to use fraction collector and Versatile Liquid Handler. An autosampler works by aspirating and injecting a precise sample volume into the mobile phase stream. You basically tell it to shut down when the pressure drops to a specified level. Properly shut down all the HPLC modules from the front (LC-20 and LC-30) or backUltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMThe PTFE frits in your Agilent LC pumps will need to be replaced from time to time as part of a routine maintenance program. 05. Purification Sample Management From HPLC fraction collection and aspiration to sample injection, Waters provides easy-to-use sample management options. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Prepare the Pump • Use fresh or different mobile phase (as required). Pump intelligence - Active Damping - with high pump resolution (~300 pL minimum step) 3. Page 70 Using the Pump Algae Growth in HPLC Systems frits to block over short-term usage. Figure 2 shows how to perform the purge for an i-Series. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. Manually prime using a priming syringe: Insert the priming syringe into the Luer fitting at the center of the draw-off valve handle. Turn on the pump flow rate to 3 mL/min through your CDS Software, Lab Advisor or through the Instant Pilot and open. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. 6 Calibration Procedure of HPLC: 6. Read Less. Remove and disassemble the pump head. Initially, the pressure was unstable. 88 Service Handbook for 1050 Series of HPLC Modules - 11/2001 Pumps: General Information Specifications Specifications Table 2 Specifications of 1050 PumpsHi,when I do wet prime/dry prime in hplc ? Thank you. Retention times can shift from one run to the next – you need to know when it is ok and when it signifies a problem. From the data analysis program vent the system. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent 1290 G4220x or G4204x Pump. 6. The historical evolution of HPLC pumps and their characteristics has been reviewed in many textbooks (1–3), as well as journal articles (4–6). Pump 15 min at this pressure to wear the seals in. 998. HPLC is an abbreviation for High Performance Liquid Chromatography. ( refer to Pump Operating Manual ). • Purge each channe lwtih 25–3m. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. 2. derivatization reagent, microdialysis liquid,…)The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. thermoscientific Vanquish Pump H VH-P10-A-02 Operating Manual 4820. Introduction 4 1. Remove and clean pistons. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. The preparative LC system performs automatic simulations using the pre-preparative results. Request Information. As above, carry out purging, cleaning, and maintenance procedures. 3 Purge the Workstation Pumps a. • Clean the bottle head assembly using lint-free wipes to minimize solvent carryover. Up to 6 solvent channels for binary high-pressure mixing. High Pressure Liquid Chromatography. Remove sample vials from the auto-sampler tray g. pump program to 90/10 water/ACN to flush the column for 15 min at 1. Fig 2. 7. solvents. 4. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. On the front panel of the pumps, press the VP button. 7:30. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. The HPLC pump, often called a solvent delivery system, provides a precise flow of mobile phases of a specified composition to the column. Mobile phase is passed through porous polymer tubing placed in a vacuum chamber. 1. Use only HPLC-grade solvents and water filtered through 0. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. This pump delivers reproducible gradients for routine HPLC or UHPLC applications with UV or MS detection where speed, resolution, and uncompromised data quality are essential. Optional solvent selection valves can be added for solvent. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). • Purge each channel for at least 10 min at 5 mL/min. The pump (s) should be run (column disconnected and bypassed) run with a solution of pure HPLC grade water to try and rinse the material off. the purge valve, and the actual pump mechanism. Search by lot number or partial lot number. Priming and Purging the 1525 Binary HPLC Pump. An optional seal wash function is available for applications usingOpen the purge valve and set the flow rate of 5 mL/min for each channel with HPLC grade water. 2 to 0. Note: A wet connection between the tubing and the column avoids any air bubbles being trapped during. 5 mL/min c. Then press “Enter”, “1”, “Enter”, “CE”, “CE”. Close prime/purge valve. Perform seal wear-in procedure. 05 0 0. Steps to follow: Store the column in an appropriate solvent (check column information sheet) When you remove the column, add a column union so the detector gets flushed as well in the following steps. 2. Run at least 25 ml buffer through degasser and pump (5 minutes) e. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. ) If system has check valve, loosen valve to allow air to escape. Note: Follow the instructions in the Ready Check Results window and close both purge screws. Prepare the Pump • Use fresh or different mobile phase (as required). Air bubbles can create such flow anomalies. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. This post continues the series of Chromatography Explained and will focus on introducing HPLC and how. 5 01. Seal wash holes Allow you to flush and clean the plunger seals. Seal could have fluoropolymer O-ring energizer instead (black O-ring). You. 0:44. 1. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA™ avant system. g. Operating principle. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. For best performance you must know your eluents 2. Pressure B. off. Priming the 1525 Binary HPLC Pump Using Empower When to Prime Priming and purging is necessary to ensure proper pump operation when: • You start the pump for the first time • You change eluent • The system has been idle for some time Parts Required None Tools/Materials Required • Priming syringe (in Startup Kit; WAT010337) Procedure 1. Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. If only an occasional bubble is present, the flow rate will be erratic, causing retention time problems. e. It is column chromatography. Changing from one operating pump head to the other you simply move the yellow connection tube to the other pump head outlet. g. Open the Purge Valve by turning it counterclockwise b. Priming and Purging the 1525 Binary HPLC Pump. 5. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. Make sure that you know which solvent is linked to which pump. ANSWER: The purge solvent is the solvent being pushed from the pump. Close the purge valve. This pump enables quaternary solvent delivery at pressures up to 800. Make sure that you know which solvent is linked to which pump. 5:26. During step 1, check that the solvent moves into the solvent line correctly and it goes out the Purge Valve. This sets the mobile phase composition to 100% water so we can purge the water line. Change the gradient. Non-circular drive gears provide smooth, uniform eluent delivery. Newsroom; Company Information;HPLC Setup Section 6. Purge valve Purge functionFig. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. Relevant functions marked for purging, flow settings ect. Among the various technologies developed for chromatography,. c. The high-precision P680 HPLC pumps are high-quality modules designed for HPLC analysis as part of a Dionex Summit HPLC system. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. A key element of its user-friendly functionality is the accessibility of the system for. 2. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then toHPLC, and biocompatible pumps. This is needed to purge any air from the tubing, introduce fresh mobile phase to. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. 1-15 of 16. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is zero; Analysis. Related: 6. e. Perform search. If the solvent line is "dry," perform "dry" primes by opening the manual prime and purge valve, and draw out 10 mL from each solvent line using a syringe. 23 MPa. 1–1% solution by volume is sufficient. methanol and purge the autosampler with methanol for at least 20 minutes. This small piece is meant to protect the rest of the system from particulates, and it needs to be replaced regularly. Set a flow of 5 mL/min and purge for 5 min. Performing the Ramp and Decay Test After a PM. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. When you vent the system, the components are turned off and the rough pump turns off. Use solvent inlet filters to protect the system from incoming particles. Attach syringe to prime/purge valve. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. If still you have the same problem, checkout the rest of tubing. When you vent the system, the components are turned off and the rough pump turns off. 1290 Infinity II High Speed Pump User Manual. This video shows the operation a HPLC purge valve. HPLC Pumps. Detector – responds to the separated analytes emerging from the HPLC. If the pressure reaches its maximum (press. 3. Overlay of 6 pump proportioning chromatograms in 10% gradient. Inside the purge valve is a small piece of polytetrafluoroethylene, called a PTFE frit. The impact of this type of clogging will be gradual, not sudden. • Syringe, loop & injector plunger seal. Santa Clara, CA 95051. 0:44. Each page is marked with a tab and a footer to help you access information. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. 5 Close the purge valve, set the flow to a rate adequate to achieve a pressure of 350 bar. The manual covers installation, operation, maintenance, and troubleshooting of the pump. ), prime system if necessary. 7 Repeat steps 4-6 with the next 3 valves. 2. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersChromaster HPLC 5110 pump with auto-purge valve. , 10 mL/min. Flush the column with 10/90 water/ACN for 5 min at 1. Kata kunci / keywords: HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product. Seat backfl ush is enabled by a second pump, an integrated high-pressure fl ush pump. Insert the suction tube for the flow channel to be purged into the reservoir bottle containing the mobile phase and place the bottle on the reservoir tray. Purification Solvent Management. It accommodates flow rates from 0. 1ml/min2 g. 6. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. 5301 Stevens Creek Blvd. ID stainless steel tubing kit • Galaxie Chromatography Software-0. Using a solvent filtration kit or an online degasser will remove any dissolved. 3:36. g. 1. You may find that the manufacturer of your particular. In stainless steel (SS) the passivation process uses nitric acid or citric acid to remove free iron from the surface. 물론 이건 내 생각이다! 여튼 purge. c Enter the new parameters in step 8, and click OK. 02 in. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. Problem 3:Inconsistent or too slow pump flow rate. g. Purging pump and autosampler for i-Series Step 4. Set the pump to 100% 'A' at 1. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. Currently loaded videos. It features an integrated pressure sensor, which ensures pressure-independent flow rate accuracy. The quantity is such that the compression ratio of the pump is reduced to 10:1 max. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. If problem. 3. purge is complete, enter a flow of 1 mL/min and 100% B. Over time, these frits will accu. What is purging in HPLC? Answer: “When setting up an HPLC system, the aim of the purge is simply to flush through all the lines so that any remaining solvent in them from a previous analysis or wash is replaced with the new mobile phase. The PTFE frit is very accessible and easy to switch out, too. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. 2HPLC is a High Performance liquid Chromatography. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. If you have a pump with an automatic purge valve, read the note below the article. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. Run through the prime purge valve first (10 mls), then. Air bubbles Filter using a 0. close the purge. The name of the game in HPLC is repeatability. 3 Page 5 Column Observations Potential Problems[][]Purge the pump with fresh diluent (filtered and degassed). Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors. Purge the pumps each time fresh eluent or water is prepared. New InletWeaver using proven Jet Weaver technology The 1290 Infinity Quaternary Pump offers the highest accuracy andAir or particulates in head of pump or valves of the pump Leakage in transmission lines Solution • Avoid formation of negative pressure, utilisation of equalising valves in the caps of the eluent flask, prepare fresh buffer • Check tightness, if necessary, replace seals or valves • Purge the pumps with water or IPA, flush waterFirmenich Dubai. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. 322 HPLC PumP | uSER’S GuIDE 13 De SC r I pt ION pUMp heaDS Two interchangeable pump head types are available:Passivation is a chemical treatment of stainless steel and purging is a cleaning process. Once the system is purged, set the flow rate at 0. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. g. 4. *Run to run RT variation of ±0. TapIn this technical article you will learn about the procedure for purging air from a reversed-phase column.